Protein Biochemistry and Proteomics
The main focus of the workplace is mass spectrometry in protein analysis. Research staff have access to equipment for protein and peptide separation supplemented with mass spectrometers with MALDI and ESI ionization. Scientists use common methods for sample preparation and purification, routine protein identification (peptide mapping and peptide sequencing), relative and absolute quantification, and analysis of post-translational modifications (glycosylation, phosphorylation). Their range of activities is wide: from analysis of one selected protein to proteome mapping. Intact cell mass spectrometry and spore help to identify microorganisms. Enzymes, especially of plant and microbial origin, such as dehydrogenases, oxidases and proteases, are also studied at the department. These enzymes are obtained by isolation from their natural sources (tissues, cells) or via recombinant expression of the respective genes in host microorganisms. Their studies include the description of molecular properties, the identification of typical substrates and inhibitors, eventually crystallization and structural analysis. Researchers also look for practical use of enzymes in food analysis by the construction of biosensors or the immobilization of enzymes on solid carriers. Research into metabolic pathways for biosynthesis and biodegradation of certain metabolites (e.g. plant hormones) is also one of the research directions of this department.